444 - Clinical Effect of a Cannabidiol-Based Mouthrinse on Dental Enamel

Purpose: This study evaluated the in situ antibiofilm efficacy of experimental mouthrinses containing cannabidiol (CBD) and their effects on the physical and mechanical properties of dental enamel.

Methods: Bovine enamel fragments were mounted in intraoral appliances worn by 14 participants in a double-blind, randomized cross-over design. Mouthrinses containing CBD at 0,01%, 0,05%, and 0,1%, placebo, and 0,12% chlorhexidine (CHX) were tested. One side of each appliance was exposed to a 20% sucrose solution. After 7 days, surface roughness (Ra), relative microhardness, and color stability (ΔE00) were measured before and after treatments. Biofilm and yeast formation (log10 CFU) were also quantified. Quantitative data were analyzed using two-way ANOVA with Bonferroni post hoc tests (P < .05), and microbial data were evaluated by Kruskal-Wallis followed by Dunn’s test (P < .05).

Results: Sucrose did not significantly affect Ra (P>.05), and only CBD0,1% produced higher roughness than CHX under sucrose exposure (P < .05). Relative microhardness (%) did not differ among treatments (P>.05); however, sucrose reduced microhardness for placebo and CBD0.01% (P < .05). CHX exhibited the highest ΔE00 values, significantly greater than all other treatments (P < .05), exceeding perceptibility (0.8) and acceptability (1.8) thresholds, while sucrose had no effect (P>.05). Biofilm formation was similar among CHX, CBD0.05%, and CBD0.1% (P>.05), although CBD0.01% showed higher values than CHX with sucrose (P < .05). CHX resulted in lower yeast counts than CBD0.01% and CBD0.1% (P < .05).

Conclusion: Mouthrinses containing CBD0.05% showed potential for reducing dental biofilm without causing significant adverse effects on enamel properties.